Analyse Pestizidrückstände
Flüssigchromatographie
Device name:LC-MS/MS (QTRAP 6500+)
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Description: Triple-stage quadrupole/ion trap LC-MS/MS instrument for ESI/APCI measurements in positive and/or negative mode
The combination of the Agilent 1290 Infinity II HPLC with the Sciex Triple Quadrupole QTRAP 6500+ mass spectrometer forms a high-performance system for the sensitive analysis of environmental contaminants in complex matrices (e.g., water, sediment, animal matrices, plants). The HPLC ensures excellent chromatographic separation thanks to its high pressure stability of up to 1300 bar. The Sciex 6500+ mass spectrometer utilizes IonDrive™ technology, electrospray ionization (ESI), or atmospheric pressure chemical ionization (APCI) to achieve detection limits in the low pg range, which is particularly important for trace-level environmental analysis. The Analyst software is used for instrument control, method development, data acquisition, and processing. Identification and quantification of analytes in the samples are performed using the Sciex OS software.
The analytical system is used to determine and identify active substances and their metabolites. In this context, multi-method approaches are playing an increasingly crucial role, meaning that not only individual substances but also mixtures of substances in environmental or laboratory samples are analyzed to obtain insights into the fate and behavior of environmental contaminants (e.g., pesticides, rodenticides, emerging contaminants) in the agricultural ecosystem. Furthermore, these analytical results can also help address questions in the fields of ecotoxicology, resistance and climate research.
Analyse Pestizidtoxität
Aktivitätsmessung Wassertiere
Device name:Multispecies Freshwater Biomonitor
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Description: information will be provided soon
Analyse von Algen
Algenanalyse in Süßwasser
Device name:AlgaeLabAnalyzer bbe Moldaenke
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Description: information will be provided soon
Analyse von Naturstoffen
Flüssigchromatographie (für Sekundärstoffwechselmetabolite, polare Metabolite und Lipide)
Device name:HPLC/DAD/ELSD
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Description: Agilent 1100 high-performance liquid chromatography system consisting of a degasser (Jasco DG-2080-54), a binary pump (G1312A), an autosampler (G1329A, 100 µL sample loop), an autosampler thermostat (G1330A) and a column oven (G1316A) in series with a diode array detector (G1315A) and a light scattering detector (ELSD, SEDERE-SEDEX75). Various normal-phase and reversed-phase columns are available for method development.
Applications: targeted analysis and absolute quantification of non-volatile metabolites across different polarity ranges, with moderate sensitivity up to the upper ppm range (DAD) or low sensitivity (ELSD) down to the lower percentage range. Analytical methods for the analysis of carotenoids, C17-polyacetylenes, anthocyanins, purine alkaloids, hydroxycinnamic acid conjugates, flavonoids, flavonoid aglycones (after acidic/enzymatic hydrolysis of glycosylated flavonoid conjugates), desulfo-glucosinolates (after enzymatic desulfation of glucosinolates) and fructooligosaccharides are available.
For absolute quantification, reference compounds are required to calibrate the detector (DAD, ELSD). Without a calibrated detector, only the relative concentrations of a metabolite can be compared between different samples.
Device name:HPLC/DAD/FLD
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Description: Agilent 1100 high-performance liquid chromatography system consisting of a degasser (ERC-3415), a binary pump (G1312A), an autosampler (G1329A, 100 µL sample loop), an autosampler thermostat (G1330A) and a column oven (G1316A) in series connection with a diode array detector (G1315A) and a fluorescence detector (G1321A). Various normal-phase and reverse-phase columns are available for method development.
Applications: targeted analysis and absolute quantification of non-volatile metabolites across different polarity ranges, with moderate sensitivity up to the upper ppm range (DAD) or high sensitivity (FLD) down to the lower ppm range. Analytical methods are available for the analysis of carotenoids, C17-polyacetylenes, anthocyanins, purine alkaloids, hydroxycinnamic acid conjugates, flavonoids, flavonoid aglycones (after acidic/enzymatic hydrolysis of glycosylated flavonoid conjugates), and desulfo-glucosinolates (after enzymatic desulfation of glucosinolates).
For absolute quantification, reference compounds are required to calibrate the detector (DAD, FLD). Without a calibrated detector, only the relative concentrations of a metabolite can be compared between different samples.
Device name:UHPLC/DAD/API-QTOFMS
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Description: Agilent 1290 ultra-high-performance liquid chromatography system consisting of a binary pump (G4220A), an autosampler (G4226A, 20 µL sample loop), an autosampler thermostat (G1330B) and a column oven (G1316C) in series with a diode array detector (G1316C) and an Agilent atmospheric pressure ionization quadrupole time-of-flight mass spectrometer (G6550A). A dual Agilent Jet Stream ES and an APCI source are available as ion sources. Furthermore, various chromatographic columns are available for the separation of polar (HILIC), semi-polar (RP-C18), and non-polar compounds (RP-C8).
Applications: targeted and non-targeted profiling of non-volatile metabolites across different polarity ranges, e.g., plant and fungal secondary metabolites such as flavonoids, polyketides, phenylpropanoids, alkaloids, glucosinolates, saponins, small peptides, as well as lipids and polar compounds from primary metabolism, such as amino acids.
Depending on the analyte, high sensitivity down to the low ppm range is achieved. In unfractionated total extracts of plant tissues, this technique can detect and relatively quantify several hundred metabolites. To annotate the detected compounds, the elemental composition can be determined. Partial structural characterization can be performed by interpreting high-resolution collision-induced dissociation spectra recorded in separate tandem mass spectrometry experiments. Typically, 5–10% of the detected compounds can be structurally annotated.
As with other non-targeted MS-based profiling methods, only the relative levels of a metabolite can be compared across different samples, but not the levels of different metabolites within a single sample. Absolute quantification can only be performed for known compounds for which a reference compound is available.
Quantitative metabolite profiling involves strict requirements regarding experimental design (replication, randomization, blocking, balancing). It is often not productive to analyze samples that have already been collected. Therefore, please plan such measurements with us before the start of the project or when submitting a proposal so that we can design an experimental setup that meets the requirements.
Analyse von Naturstoffen
Gaschromatographie (Zentralstoffwechselmetabolite, volatile und verdampfbare Metabolite)
Device name:AGILENT GC-Q-MS (F)
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Description: AGILENT 6890N gas chromatograph (GC) hyphenated with an AGILENT flame ionization detector (FID) and AGILENT 5973 single quadrupole mass spectrometer (MS). Equipped with a GERSTEL autosampler for liquid injection, solid phase micro extraction (SPME) and thermal desorption (TDU). Suitable for the quantitative and qualitative analysis of volatiles and vaporisable compounds. Comprehensive determination of maximal 300 known and unknown compounds from extracts, essential oils or headspaces (plant, microbial, and insect metabolites). The thermal desorption unit allows for the analysis of in the field collected volatiles using GERSTEL desorption liners or twister bars. An analysis takes 30-60 minutes. For metabolite profiling of very complex samples, the LECO GC-TOF-MS is the more appropriate instrument.
Device name:AGILENT GC-Q-MS (G)
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Description: AGILENT 6890N gas chromatograph (GC) hyphenated with an AGILENT flame ionization detector (FID) and AGILENT 5973 single quadrupole mass spectrometer (MS). Equipped with a GERSTEL autosampler for liquid injection and solid phase micro extraction (SPME). Suitable for the quantitative and qualitative analysis of volatiles and vaporisable compounds. Comprehensive determination of maximal 300 known and unknown compounds from extracts, essential oils or headspaces (plant, microbial, and insect metabolites). An analysis takes 30-60 minutes. For metabolite profiling of very complex samples, the LECO GC-TOF-MS is the more appropriate instrument.
Device name:GC-FID (B)
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Description: AGILENT 7890A gas chromatograph coupled with an AGILENT flame ionization detector (FID) for liquid injection and solid phase micro extraction (SPME), quantitative/compositional analysis of volatiles or vaporisable metabolites. Typically, a few known major compounds from extracts, essential oils or head space, for which authentic standards are commercially available, are determined. One analysis takes 30-60 minutes.
Device name:GC-FID (C)
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Description: AGILENT 6890N gas chromatograph coupled with an AGILENT flame ionization detector (FID) for liquid injection and GERSTEL solid phase micro extraction (SPME), quantitative/compositional analysis of volatiles or vaporisable metabolites. Typically, a few known major compounds from extracts, essential oils or head space, for which authentic standards are commercially available, are determined. One analysis takes 30-60 minutes.
Device name:GC-FID (D)
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Description: AGILENT 6890N gas chromatograph coupled with an AGILENT flame ionization detector (FID) for liquid injection and GERSTEL solid phase micro extraction (SPME), quantitative/compositional analysis of volatiles or vaporisable metabolites. Typically, a few known major compounds from extracts, essential oils or head space, for which authentic standards are commercially available, are determined. One analysis takes 30-60 minutes.
Device name:GC-FID/ODP (A)
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Description: AGILENT 6890N gas chromatograph coupled with an AGILENT flame ionization detector (FID) and a GERSTEL olfactory detection port (ODP) for liquid injection and solid phase micro extraction (SPME), quantitative/compositional analysis of volatiles or vaporisable metabolites. Typically, a few known major compounds from extracts, essential oils or head space, for which authentic standards are commercially available, are determined. One analysis takes 30-60 minutes. The olfactory port allows for human nose sensory rating of chromatographed compounds.
Analyse von Naturstoffen
Insektenbiotests von Naturstoffen
Device name:Ethovision XT Verhaltensaufnahme-System
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Description: Ethovision XT behaviour recording system, Biotest - behavioural experiments with camera system and infrared light table, examination of the reaction of insects to volatile compounds (single substances, mixtures, plant, insect and substrate odors), plant structures or leaf surfaces. Examination of the influence of pesticides or biopesticides on water or soil organisms. An animal or is filmed within a defined area. The reaction to different stimuli can be analysed via the behaviour and movement pattern.
Device name:Lokomotions - Kompensator (Kramer-Kugel)
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Description: Locomotions - compensator (Kramer Sphere), bioassay - behavioural tests on a rotating sphere with a diameter of 30 cm, checking the response of insects to volatiles (single compounds, mixtures, plant, insect and substrate odors) and optical or acoustic signals based on the movement behavior.
Analyse von Naturstoffen
zerstörungsfreie Methoden/ Schwingungsspektroskopie
Device name:Bruker Alpha ATR-FTIR
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Description: Attenuated total reflectance Fourier-transform infrared spectrometer ALPHA-P from Bruker Optics, two acquisition methods obtainable -singe reflectance (diamond, 375-7,500cm-1) or multi reflectance module (6fold, ZnSe, 600-7,500cm-1), fast and nondestructive analysis of monolayer of powdered, solid, liquid samples or films, e.g. plant material (leafs, pollen, flowers, seeds, stem, bark, root, intact or powdered), oils, extracts or fluids, pure reference compounds for structure identification, mostly applied for metabolic pre-screening of genetic resources (fennel, black cumin, allium...) development of quantification models for valuable plant ingredients (ess. oils in medicinal and aromatic plants, quantification of alkaloids in Solanaceae, polyphenols in Fabaceae), compound identification (e.g. polymers for micro plastic analysis), material authentication, process and reaction control (enzymatic digestion of phaselic acid in clover), chemical phenotyping.
Device name:BRUKER Bravo mobiles Raman-Spektrometer
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Description: hand-held Raman Spectrometer "Bravo" Bruker Optik GmbH with patented fluorescence mitigation SSE™(Sequentially Shifted Excitation) enables measurements of a wide range of materials for e.g. authentication. It features Duo LASER™ excitation using two lasers with different wavelengths resulting in high sensitivity across the largest spectral range. Exchangeable measurement tips allow analysis of different sample types like solids, liquids or extracts, powders and films. It is used for sample authentication (e.g. polymers and plastic particles > 1 mm, phenotyping of plant material...), quantification (essential oil components, fatty oils), surveillance of processes (distillation process of plant material, growing of plants).
Device name:BRUKER FT-Nahinfrarot (NIR)-Spektrometer Multi Purpose Analyser (MPA)
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Description: FT-near infrared (NIR) spectrometer "Multi Purpose Analyser (MPA)" Bruker Optics is an easy to use QA/QC instrument used for developing quantification methods or process control. Different sampling accessories are provided - sample compartment, integrating sphere and transmission unit. It is used for the analysis of liquids, semi-solids, solids, powders, intact leaves, intact insect organisms…Sample amount can range from < 1g to 25 g or single seed analysis. Routine measurements are performed in about 30 sec. allowing non-destructive high throughput analysis e.g. for single plant analysis in breeding experiments.
Device name:FT-Raman-Spektrometer RFS 100/S Bruker Optik GmbH
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Description: FT-Raman spectrometer RFS 100/S Bruker Optics, 1064 nm excitation wave length, liquid N2-cooled detector, application of solid (plants, fruits, seeds, roots, reference compounds) and liquid material (extracts, essential oils, fluids, aqueous solutions). applicability of Raman spectroscopy of plant material strongly depends on Raman activity of appropriate analytes and its concentration. Fluorescence (usually high in colored samples) can reduce spectral quality. Partly automated measurements (comparable to auto sampler) are possible using mapping tool. Measurements periods range from seconds (single point measurement) to hours (mapping experiments).
Analyse von Schwermetallen und Pflanzennährstoffen
Quantitative Elementanalyse
Device name:C/N Analyzer
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Description: Element analyzer vario EL cube by Elementar Analysensysteme GmbH: Quantitative analysis of total carbon (Ctotal), organic carbon (Corg), and total nitrogen (Ntotal) in soil and plant samples for determining the C/N ratio in environmental samples.
Device name:Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES)
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Description: ICP-OES ICAP 7600 Duo, Thermo Scientific: Quantitative multi-element method for the simultaneous determination of up to 25 elements (nutrients and trace elements, e.g. heavy metals) across a wide range of concentrations (% to ppb) in a variety of sample matrices (soil, sediment, plant, water, animal organisms such as earthworms, bees, etc.).
